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Related Concept Videos

The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

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Related Experiment Video

Updated: Jun 12, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Cyclin regulation by the s phase checkpoint.

Gloria Palou1, Roger Palou, Angel Guerra-Moreno

  • 1Biophysics Unit, Department of Biochemistry and Molecular Biology, School of Medicine, and Center for Biophysic Studies, Universitat Autonoma de Barcelona, Bellaterra, Catalonia, Spain.

The Journal of Biological Chemistry
|June 12, 2010
PubMed
Summary

The S phase checkpoint in yeast responds to DNA damage by stabilizing Clb6 cyclin levels, a process dependent on Mec1 and Rad53 kinases. This stabilization of Clb6 is crucial for cell cycle regulation under stress.

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Last Updated: Jun 12, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
10:54

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Published on: September 17, 2012

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Eukaryotic cells possess an S phase checkpoint for DNA damage and replication stress.
  • This checkpoint arrests cell cycle progression to protect DNA replication.
  • S phase cyclins Clb5 and Clb6 activate Cdc28 kinase, driving S phase onset and progression.

Purpose of the Study:

  • To investigate the regulation of S phase cyclins Clb5 and Clb6 under genotoxic stress.
  • To elucidate the mechanisms by which Clb5 and Clb6 respond to DNA damage and replication stress in Saccharomyces cerevisiae.

Main Methods:

  • Analysis of Clb5 and Clb6 protein levels and stability under genotoxic stress conditions.
  • Investigation of the role of checkpoint kinases (Mec1, Rad53, Dun1) in cyclin regulation.
  • Assessment of the involvement of transcription factors (MBF) and de novo protein synthesis.

Main Results:

  • Clb6 protein is stabilized during replication stress or DNA damage, dependent on Mec1 and Rad53 kinases.
  • Clb6 stabilization requires ongoing protein synthesis and Mlu1 cell cycle box-binding factor (MBF) activity.
  • An unstable pool of Clb5 is also stabilized under replication stress, independently of MBF and de novo synthesis.

Conclusions:

  • Clb5 and Clb6 cyclins exhibit differential regulation in response to genotoxic stress.
  • Checkpoint kinases Mec1 and Rad53 play a critical role in stabilizing Clb6.
  • These findings highlight distinct mechanisms for managing S phase cyclins during cellular stress.