Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 12, 2026

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
09:16

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

Immune activation in retinal aging: a gene expression study.

Mei Chen1, Elizabeth Muckersie, John V Forrester

  • 1Centre for Vision and Vascular Science, School of Medicine, Dentistry, and Biomedical Sciences, Queen's University Belfast, Belfast, Northern Ireland, United Kingdom.

Investigative Ophthalmology & Visual Science
|June 12, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term metabolic-adiposity burden and cardiovascular risk: the cumTyG-WHtR in Chinese adults.

BMC cardiovascular disorders·2026
Same author

Macular fibrosis secondary to neovascular age-related macular degeneration: from clinic to biology.

Progress in retinal and eye research·2026
Same author

Myeloid Cell Protein Tyrosine Phosphatase 1B Drives Retinal Neurodegeneration in Diabetic Mice.

Diabetes·2026
Same author

MSC-EVs attenuate subretinal fibrosis in choroidal neovascularization through miR-21-5p-mediated inhibition of EMT and MMT and suppression of inflammation.

Journal of neuroinflammation·2026
Same author

BLIMP1 shapes germinal center B cell clonal diversity by gating chromatin accessibility during light-to-dark zone transition.

Nature immunology·2026
Same author

Effects of High Temperature and Nitrogen Fertilizer on the Carbon and Nitrogen Metabolism Characteristics of Rice Varieties with Differing Taste Stability.

Plants (Basel, Switzerland)·2026
Same journal

Loss of ATRAID in Late-Onset, Non-Syndromic Retinitis Pigmentosa.

Investigative ophthalmology & visual science·2026
Same journal

Whole-Exome Analysis Identifies Candidate Genes Associated With Diabetic Retinopathy.

Investigative ophthalmology & visual science·2026
Same journal

Antiseptic Efficacy and Ocular Surface Toxicity of Povidone-Iodine and Chlorhexidine for Endophthalmitis Isolates.

Investigative ophthalmology & visual science·2026
Same journal

Ocular IL-1α/IFN-γ Delay the Corneal Wound Healing in Rheumatoid Arthritis Through Mitochondrial Dysfunction and NLRP3 Inflammasome Activation.

Investigative ophthalmology & visual science·2026
Same journal

New Guidelines for Ophthalmic Genetic Studies.

Investigative ophthalmology & visual science·2026
Same journal

Genomic Copy Number Variants Associated With Strabismus and Amblyopia in the All of Us Research Program.

Investigative ophthalmology & visual science·2026
See all related articles

Retinal aging in mice involves significant changes in gene expression, particularly in immune and stress response pathways. This suggests a chronic, low-grade inflammatory state characterizes the aging mouse retina.

Area of Science:

  • Ophthalmology
  • Immunology
  • Genetics

Background:

  • Retinal aging is a complex process with poorly understood molecular mechanisms.
  • Gene expression profiling offers a powerful approach to identify age-related molecular changes in the retina.

Purpose of the Study:

  • To investigate age-related alterations in gene expression within the mouse retina.
  • To identify specific molecular pathways and genes affected by retinal aging.

Main Methods:

  • Microarray analysis of neuroretinal RNA from young (3-month-old) and old (20-month-old) mice.
  • Statistical analysis using empiric Bayes shrinkage-moderated t-statistics to identify differentially expressed genes (fold change >2, P < 0.01).
  • Verification of gene expression by real-time PCR and immunohistochemistry; pathway analysis using DAVID-2008.

Related Experiment Videos

Last Updated: Jun 12, 2026

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
09:16

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

Main Results:

  • A total of 298 genes were upregulated and 137 downregulated in aging retinas.
  • Genes associated with immune responses (Ir) and tissue stress/injury (TS/I) were significantly modified.
  • Increased microglial activation and complement cascade activity were observed, with calcitonin receptor (Calcr) being the most upregulated gene (>40-fold).

Conclusions:

  • Retinal aging is characterized by the activation of gene sets involved in local inflammatory responses, termed para-inflammation.
  • The innate immune system plays a crucial role in these age-related inflammatory changes.
  • Upregulation of Calcr in aging retinas likely reflects this chronic inflammatory and stress response.