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Centrosome Duplication

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

Centrosome Duplication

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...

Centrioles and Centrosomes

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

The Cell Cycle Control System

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Dictyostelium centrin B localization during cell cycle progression.

Sebastian Mana-Capelli, Ralph Gräf, Denis A Larochelle

Communicative & Integrative Biology

|June 12, 2010

Summary

This summary is machine-generated.

This study characterizes Dictyostelium discoideum centrin (DdCenB), revealing unique cell cycle localization patterns. DdCenB is found at the nuclear envelope during interphase but not the centrosome, suggesting novel roles in cell division.

Keywords:

centrin centrosome cytokinesis dictyostelium mitosis nuclear envelope

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Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

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Published on: December 5, 2017

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Centrins are conserved calcium-binding proteins involved in diverse cellular processes.
Dictyostelium discoideum provides a model system for studying cell cycle regulation and cytoskeletal dynamics.
Novel centrins may exhibit unique localization and functions distinct from well-characterized homologs.

Purpose of the Study:

To characterize the novel centrin DdCenB from Dictyostelium discoideum.
To investigate the cell cycle-dependent localization of DdCenB.
To explore potential roles of DdCenB beyond canonical centrin functions.

Main Methods:

Sequence and phylogenetic analysis to confirm DdCenB as a centrin.
Fluorescence microscopy to visualize DdCenB localization during interphase, mitosis, and cytokinesis.
Analysis of DdCenB distribution within the nucleus and cytoplasm.

Main Results:

DdCenB localizes to the nuclear envelope during interphase.
DdCenB disappears from the nuclear envelope during mitosis and cytokinesis.
Unlike other centrins, DdCenB shows no centrosomal localization.
Cytoplasmic localization of DdCenB increases during mitosis and cytokinesis.

Conclusions:

DdCenB exhibits unusual cell cycle localization distinct from typical centrins.
The unique localization suggests DdCenB may play specialized roles in Dictyostelium cell division.
Further research is needed to elucidate the precise functions of DdCenB in cell cycle progression.