Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Generation and Characterization of a Mouse Embryonic Stem Cell Line with Psmb9 Immunoproteasome Gene Knockout.

Acta naturae·2025
Same author

THE SHORTENED ISOFORM OF a-TUBULIN IS DETECTED IN COMPLEX WITH PROTEASOMES.

Tsitologiia·2018
Same author

Proteasomes in Protein Homeostasis of Pluripotent Stem Cells.

Acta naturae·2017
Same author

[Mass spectrometric analysis of proteasomes affinity puirified from the human myelogenous leukemia cells K562].

Bioorganicheskaia khimiia·2015
Same author

[Extracellular proteasomes purification methods and the evaluation of proteasomal peptidase activities].

Tsitologiia·2015
Same author

[Proteasomes and their role in the extracellular space].

Tsitologiia·2014

Related Experiment Video

Updated: Jun 12, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants
07:43

Assaying Proteasomal Degradation in a Cell-free System in Plants

Published on: March 26, 2014

[Proteasomes: their role in cellular processes].

A S Tsimokha

    Tsitologiia
    |June 15, 2010
    PubMed
    Summary

    The ubiquitin proteasome system, a key cellular pathway, regulates protein levels and cell functions. This review details proteasome structure, diverse activities, and roles in cell cycle, DNA repair, and immunity.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Molecular Biology

    Context:

    • The ubiquitin proteasome system (UPS) is crucial for cellular protein homeostasis.
    • 26S proteasomes, the primary degradation machinery, consist of a 20S catalytic core and regulatory complexes.
    • Eukaryotic proteasomes exhibit diverse activities beyond proteolysis, including endoribonuclease, chaperone, and DNA helicase functions.

    Purpose:

    • To provide a comprehensive review of proteasome structure and function.
    • To elucidate the role of the UPS in fundamental cellular processes.
    • To highlight the regulatory mechanisms governing proteasome activity.

    Summary:

    • Proteasomes are essential for degrading regulatory proteins, controlling their cellular levels.
    • The UPS is indispensable for cell survival, regulating processes like cell cycle, transcription, apoptosis, differentiation, DNA repair, and immune response.

    More Related Videos

    Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
    09:57

    Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

    Published on: December 17, 2016

    Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
    09:25

    Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

    Published on: May 21, 2019

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    Assaying Proteasomal Degradation in a Cell-free System in Plants
    07:43

    Assaying Proteasomal Degradation in a Cell-free System in Plants

    Published on: March 26, 2014

    Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
    09:57

    Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

    Published on: December 17, 2016

    Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
    09:25

    Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain

    Published on: May 21, 2019

  • Proteasomal populations are heterogeneous and subject to complex post-translational modifications.

    • Impact:

    • Understanding proteasome function is vital for comprehending cellular health and disease.
    • This review consolidates current knowledge on proteasome involvement in diverse biological pathways.
    • Insights into proteasome regulation may offer therapeutic targets for various diseases.