Related Experiment Video
Updated: Jun 12, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Mechanism and engineering of polyketide chain initiation in fredericamycin biosynthesis
Abhirup Das1, Ping-Hui Szu, Jay T Fitzgerald
1Department of Chemistry, Stanford University, Stanford, California 94305, USA.
Abstract:
The ability to incorporate atypical primer units through the use of dedicated initiation polyketide synthase (PKS) modules offers opportunities to expand the molecular diversity of polyketide natural products. Here we identify the initiation PKS module responsible for hexadienyl priming of the antibiotic fredericamycin and investigate its biochemical properties. We also exploit this PKS module for the design and in vivo biosynthesis of unusually primed analogues of a representative polyketide product, thereby emphasizing its utility to the metabolic engineer.
More Related Videos
10:41The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
Published on: January 13, 2013
07:59A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Related Concept Videos
Cationic Chain-Growth Polymerization: Mechanism
Peptidoglycan Synthesis
Biosynthesis in Bacteria
Production of Antibiotics
Anionic Chain-Growth Polymerization: Mechanism
Inhibitors of Gram-positive Cell Wall Synthesis