Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Protein Metabolism01:21

Overview of Protein Metabolism

Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Cellular Adaptation II: Hypertrophy01:26

Cellular Adaptation II: Hypertrophy

Hypertrophy is the increase in the size of individual cells, resulting in the enlargement of a tissue or organ. Unlike hyperplasia, which involves an increase in cell number, hypertrophy is characterized by an increase in cell volume. This process often occurs in response to higher functional demand or hormonal stimulation, leading to the production of more structural proteins and organelles, thereby enhancing the cells' work capacity.There are two primary types of hypertrophy: physiological...
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
Alterations in Muscle Tone lll01:11

Alterations in Muscle Tone lll

Rigidity and myotonia are distinct abnormalities of muscle tone that affect resistance and relaxation during movement. Although both involve altered muscle contraction, they arise from different neurological and muscular mechanisms.CharacteristicsRigidity is characterized by uniform resistance to passive movement across the entire range, independent of speed, affecting flexors and extensors equally. It may appear as lead-pipe rigidity (smooth, constant resistance) or cogwheel rigidity...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reconsidering the Interpretation of Migration Percentage in Cerebral Palsy: The Role of Age-related Skeletal Maturation.

Journal of pediatric orthopedics·2026
Same author

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same author

Structural and transcriptomic alterations underlying the progression of aortic dissection in Fbn1<sup>G234D/G234D</sup> mice.

Scientific reports·2026
Same author

Exploring the Role of the Extracellular Matrix in Disease and Aging.

Juntendo medical journal·2026
Same author

A case of rapidly progressive cervical primary spinal epidural lymphoma.

Surgical neurology international·2025
Same author

Collagen XV preserves heart function and protects from pathological remodelling after myocardial infarction.

The FEBS journal·2025
Same journal

Airway macrophage specific glycocalyx expression and remodeling following viral infection.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Cartilage intermediate layer protein (CILP) in cardiac fibrosis: Protective or pathogenic?

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Polymerizing laminins: Assembly, functions and disorders.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Oligodendrocyte integrin-β1 regulates blood-brain barrier and remyelination in hemorrhagic brain.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Dynamic regulation of the tissue microenvironment by integrins and the extracellular matrix.

Matrix biology : journal of the International Society for Matrix Biology·2026
Same journal

Corrigendum to "Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro" [Matrix Biology 146 (2026) 102008].

Matrix biology : journal of the International Society for Matrix Biology·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Freezing Injury in Mouse Masseter Muscle to Establish an Orofacial Muscle Fibrosis Model
06:33

Freezing Injury in Mouse Masseter Muscle to Establish an Orofacial Muscle Fibrosis Model

Published on: December 29, 2023

Perlecan deficiency causes muscle hypertrophy, a decrease in myostatin expression, and changes in muscle fiber

Zhuo Xu1, Naoki Ichikawa, Keisuke Kosaki

  • 1Research Institute for Diseases of Old Age, Juntendo University School of Medicine, Tokyo, Japan.

Matrix Biology : Journal of the International Society for Matrix Biology
|June 15, 2010
PubMed
Summary
This summary is machine-generated.

Perlecan deficiency in mice enhances skeletal muscle hypertrophy and reduces myostatin signaling, particularly in fast-twitch fibers. This suggests perlecan plays a key role in regulating muscle mass and response to mechanical stress.

More Related Videos

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

Related Experiment Videos

Last Updated: Jun 12, 2026

Freezing Injury in Mouse Masseter Muscle to Establish an Orofacial Muscle Fibrosis Model
06:33

Freezing Injury in Mouse Masseter Muscle to Establish an Orofacial Muscle Fibrosis Model

Published on: December 29, 2023

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans
09:18

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Published on: September 7, 2021

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
07:51

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Published on: April 30, 2014

Area of Science:

  • Muscle physiology
  • Extracellular matrix biology
  • Signaling pathways

Background:

  • Perlecan is a crucial basement membrane component surrounding skeletal muscle fibers.
  • Understanding perlecan's role is vital for muscle health and disease research.
  • Myostatin signaling is a key regulator of muscle mass.

Purpose of the Study:

  • To investigate the function of perlecan in skeletal muscle hypertrophy.
  • To determine perlecan's role in myostatin signaling under mechanical stress.
  • To utilize a mouse model deficient in skeletal muscle perlecan (Hspg2(-/-)-Tg).

Main Methods:

  • Comparison of Hspg2(-/-)-Tg mice with wild-type controls (WT-Tg).
  • Analysis of muscle fiber cross-sectional area (CSA) and type composition (e.g., MHC IIb, IIx).
  • Assessment of myostatin and ALK4 expression, and Smad activation.
  • Application of mechanical overload/unload models (tenotomy) on fast (plantaris) and slow (soleus) muscles.

Main Results:

  • Perlecan deficiency led to increased fiber CSA and IIx fibers in tibialis anterior muscle.
  • Myostatin and ALK4 expression, along with Smad activation, were reduced in Hspg2(-/-)-Tg muscle.
  • Mechanical overload enhanced plantaris muscle weight gain and reduced wet weight loss during unloading in Hspg2(-/-)-Tg mice.
  • Overload effects were more pronounced with decreased myostatin expression in Hspg2(-/-)-Tg mice.
  • Soleus muscle showed no significant changes upon overloading.

Conclusions:

  • Perlecan is essential for maintaining fast muscle mass and fiber type composition.
  • Perlecan significantly influences myostatin signaling pathways.
  • Targeting perlecan may offer therapeutic strategies for muscle-related conditions.