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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
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Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

Small-molecule FRET probes for protein kinase activity monitoring in living cells

Angela Vaasa1, Marje Lust, Anna Terrin

  • 1Institute of Chemistry, University of Tartu, 50411 Tartu, Estonia.

Biochemical and Biophysical Research Communications
|June 15, 2010
PubMed

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...