Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Heat and solvent resistant electrospun nanofibers for tunable fluid transport in lateral flow applications.

Scientific reports·2026

Same author

Fast enrichment and detection of circulating tumor cells from large volumes of whole blood of breast cancer patients utilizing a functionalized bioaffinity CTC filtration membrane.

International journal of cancer·2025

Same author

Hydrogel beads for enhanced nucleic acid analysis in complex fluid matrices.

Lab on a chip·2025

Same author

Attachment of Hydrogel Patches to Eye Tissue through Gel Transfer using Flexible Foils.

ACS applied materials & interfaces·2025

Same author

Weather-responsive adaptive shading through biobased and bioinspired hygromorphic 4D-printing.

Nature communications·2024

Same author

Generation of Tailored Multi-Material Microstructures Through One-Step Direct Laser Writing.

Small (Weinheim an der Bergstrasse, Germany)·2024

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Polymer Microarrays for High Throughput Discovery of Biomaterials

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

Printed protein microarrays on unmodified plastic substrates.

Meike Moschallski¹, Johannes Baader, Oswald Prucker

¹Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Köhler-Allee 103, 79110 Freiburg, Germany.

Analytica Chimica Acta

|June 15, 2010

Summary

This study introduces a novel, single-step method for creating protein microarrays on plastic surfaces. The technique uses photochemical attachment to immobilize functional proteins within hydrogel dots, enhancing microarray production.

More Related Videos

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

Related Experiment Videos

Last Updated: Jun 12, 2026

Polymer Microarrays for High Throughput Discovery of Biomaterials

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Published on: November 5, 2016

Area of Science:

Biotechnology
Materials Science
Surface Chemistry

Background:

Immobilizing functional proteins on chip surfaces is crucial for protein microarray generation.
Existing methods face challenges in achieving efficient and stable protein attachment.

Purpose of the Study:

To present a novel, single-step method for producing protein microarrays on unmodified plastic substrates.
To demonstrate the simultaneous photochemical attachment of polymer/protein mixtures and hydrogel formation.

Main Methods:

Printing polymer/protein mixtures onto plastic substrates.
Utilizing photochemical crosslinking with benzophenone to form hydrogel dots.
Covalently immobilizing proteins within a 3D hydrogel scaffold.

Main Results:

Achieved a probe density of 4 x 10^9 protein molecules per spot.
Demonstrated high signal-to-noise ratios (>200) in antigen-binding assays.
Confirmed that immobilized antibodies retain accessibility and functionality.

Conclusions:

The developed method offers a simple and effective approach for creating protein microarrays with high probe density.
The photochemical immobilization strategy ensures protein functionality and stability on plastic surfaces.
This technique has significant potential for advancing protein microarray applications.