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Association of exogenous DNA with cattle and insect spermatozoa in vitro
P W Atkinson1, E R Hines, S Beaton
1CSIRO Division of Entomology, Canberra, NSW, Australia.
Molecular Reproduction and Development
|May 1, 1991
Abstract:
Spermatozoa isolated from domestic cattle (Bos taurus), the Australian sheep blowfly (Lucilia cuprina), and the honeybee (Apis mellifera) are capable of binding exogenous radiolabeled linear DNA. Both motile and nonmotile bovine sperm exhibit four distinct patterns of DNA association. Following treatment with DNase I, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of DNA that associates with bovine sperm may be sequestered within the sperm head.