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Related Experiment Video

Updated: Jun 12, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Whole-genome DNA methylation profiling using MethylCap-seq.

Arie B Brinkman1, Femke Simmer, Kelong Ma

  • 1Radboud University, Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Faculty of Science, 6500 HB Nijmegen, The Netherlands. a.brinkman@ncmls.ru.nl

Methods (San Diego, Calif.)
|June 15, 2010
PubMed
Summary
This summary is machine-generated.

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MethylCap-seq offers genome-wide DNA methylation profiling using MeCP2

Area of Science:

  • Epigenetics and Genomics
  • Molecular Biology

Background:

  • DNA methylation is crucial for gene regulation.
  • Accurate genome-wide methylation profiling is essential for understanding biological processes and diseases.

Purpose of the Study:

  • To describe the principles and details of the MethylCap-seq procedure.
  • To highlight its utility for genome-wide DNA methylation mapping.

Main Methods:

  • MethylCap-seq utilizes the MBD domain of MeCP2 for methylated DNA capture.
  • Elution with a salt gradient stratifies DNA by CpG density.
  • Next-generation sequencing of eluted DNA provides detailed methylation profiles.

Main Results:

  • MethylCap-seq enables cost-effective deep sequencing coverage.

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Last Updated: Jun 12, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
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Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients

Published on: June 16, 2017

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

  • The method generates detailed genome-wide maps of methylated regions.
  • It facilitates the detection of DNA methylation in known and novel regions.

    • Conclusions:

    • MethylCap-seq is a robust method for comprehensive DNA methylation analysis.
    • The procedure allows for efficient and detailed genome-wide methylation profiling.
    • It is applicable to various starting material sources.