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Detection of toxigenic Clostridium difficile in diarrheal stools by rapid real-time polymerase chain reaction
Simon D Goldenberg1, Thomas Dieringer, Gary L French
1Directorate of Infection, Guy's and St. Thomas' NHS Foundation Trust, London, UK. simon.goldenberg@gstt.nhs.uk
Abstract:
The Cepheid Xpert polymerase chain reaction assay (Sunnyvale, CA) had a sensitivity of 100%, specificity of 96.7%, and positive and negative predictive values of 90.5% and 100%, respectively, compared with toxigenic culture for the laboratory diagnosis of Clostridium difficile in diarrheal stool samples. This test appears to be a significant improvement to poorly performing enzyme immunoassays.
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