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Updated: Jun 12, 2026

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
Published on: July 10, 2021
Birth after 12 hours of oocyte refrigeration
Onder Coban1, Oguzhan Hacifazlioglu, H Nadir Ciray
1Cyprus IVF Center, Famagusta, Cyprus.
Objective:
To assess cycle outcome after oocyte refrigeration.
Design:
Case report.
Setting:
Private IVF center.
Patient(S):
One couple in a donor oocyte program.
Intervention(S):
Intracytoplasmic sperm injection and blastocyst culture after refrigeration of oocytes for 12 hours.
Main Outcome Measure(S):
Birth.
Result(S):
Fourteen two-pronuclei zygotes from 17 metaphase II refrigerated oocytes resulted in transfer of two blastocysts at day 5 and cryopreservation of six excess embryos at day 6. The patient delivered one healthy male baby after 38 weeks' gestation.
Conclusion(S):
The successful outcome of oocyte refrigeration indicates that this protocol could be useful in circumstances in which a delay in obtaining spermatozoa arises.
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