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Related Concept Videos

Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Recombinant DNA01:09

Recombinant DNA

Overview
Recombinant DNA01:09

Recombinant DNA

Overview

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Related Experiment Video

Updated: Jun 12, 2026

Recombineering Homologous Recombination Constructs in Drosophila
14:23

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Published on: July 13, 2013

Recombineering using RecTE from Pseudomonas syringae.

Bryan Swingle1, Zhongmeng Bao, Eric Markel

  • 1United States Department of Agriculture, Agricultural Research Service, 321 Plant Science Building, Tower Road, Ithaca, NY 14853, USA. Bryan.Swingle@ars.usda.gov

Applied and Environmental Microbiology
|June 15, 2010
PubMed
Summary
This summary is machine-generated.

Researchers identified Pseudomonas genes that facilitate genomic recombination of linear DNA. These genes, similar to E. coli phage proteins, enable targeted gene disruptions in Pseudomonas syringae, advancing genetic engineering capabilities.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Genomic recombination is crucial for DNA repair and genetic diversity.
  • Bacteriophage-encoded recombination systems, like lambda Red and RecET in E. coli, are well-characterized.
  • Developing efficient genetic manipulation tools for diverse bacterial species, such as Pseudomonas, remains a challenge.

Purpose of the Study:

  • To identify and characterize genes in Pseudomonas syringae that promote the genomic recombination of linear DNA.
  • To evaluate the efficiency of these Pseudomonas-encoded recombination functions in facilitating gene editing.

Main Methods:

  • Identification of candidate genes based on sequence homology to known phage recombination proteins (lambda Red, RecET).
  • Testing the recombination activity of identified Pseudomonas genes in P. syringae pv. tomato DC3000 using electroporation and a quantitative recombination assay.
  • Assessing the roles of homologous RecT and RecE proteins in single-stranded and double-stranded DNA recombination.

Main Results:

  • The Pseudomonas RecT homolog alone can promote the recombination of single-stranded DNA oligonucleotides.
  • Efficient recombination of double-stranded DNA requires the co-expression of both Pseudomonas RecT and RecE homologs.
  • The identified recombination system was successfully applied to create targeted gene disruptions in the P. syringae chromosome.

Conclusions:

  • Pseudomonas syringae possesses functional homologs of phage-mediated recombination systems.
  • The identified Pseudomonas recombineering system, utilizing RecT and RecE homologs, is effective for targeted gene modification.
  • This study provides a valuable tool for genetic engineering in Pseudomonas species.