Hansschlegelia zhihuaiae sp. nov., isolated from a polluted farmland soil

A novel Gram-negative, aerobic, coccoid-shaped strain designated S 113(T) was isolated from a polluted-soil sample collected in Jiangsu Province, China. A polyphasic taxonomic study including phylogenetic analysis based on the 16S rRNA gene sequence and determination of phenotypic characteristics was performed on the new isolate. The highest 16S rRNA gene sequence similarity was 96.8 %, with Hansschlegelia plantiphila S(1)(T). The predominant respiratory quinone was ubiquinone 10 (Q-10). The major fatty acids were C(18 : 1)ω7c and C(16 : 0). The G+C content of the DNA was about 65.7 mol%. DNA-DNA hybridization experiments showed 44.9 % relatedness for strain S 113(T) with its closest relative, H. plantiphila NCIMB 14035(T). The dominant phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylcholine. The results of our polyphasic taxonomic analysis indicate that strain S 113(T) represents a novel species within the genus Hansschlegelia, for which the name Hansschlegelia zhihuaiae sp. nov. is proposed. The type strain is S 113(T) ( = DSM 18984(T)  = CCTCC AB 206143(T)  = KCTC 12880(T)).