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Related Experiment Video

Updated: Jun 12, 2026

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Three Different Protocols of Corneal Collagen Crosslinking in Keratoconus: Conventional, Accelerated and Iontophoresis

Published on: November 12, 2015

Corneal collagen cross-linking.

Mirko R Jankov Ii¹, Vesna Jovanovic, Ljubisa Nikolic

¹Laser Focus-Centre for Eye Microsurgery.

Middle East African Journal of Ophthalmology

|June 15, 2010

Summary

This summary is machine-generated.

Corneal collagen cross-linking (CXL) strengthens corneas using riboflavin and UVA light. This safe procedure inhibits ectasia progression and aids in treating ulcers and post-surgical issues.

Keywords:

Corneal Collagen Cross-Linking Corneal Ectasia Corneal Ulcer Intracorneal Ring Segments Keratoconus Topography-Guided Photoablation

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Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network

Preparing Porcine Eyes for Confocal Reflectance Microscopy to Visualize the Vitreous Collagen Fiber Network

Published on: October 17, 2025

Area of Science:

Ophthalmology
Biomaterials Science
Corneal Surgery

Background:

Corneal collagen cross-linking (CXL) utilizes riboflavin and ultraviolet-A (UVA) to enhance corneal biomechanical strength.
This process involves photosensitized oxidation, increasing intra- and interfibrillar covalent bonds.

Purpose of the Study:

To detail the mechanism and effects of corneal collagen cross-linking (CXL).
To outline indications for CXL, including corneal ectasias and infectious ulcers.
To review safety considerations and combination therapies.

Main Methods:

Application of riboflavin solution followed by UVA irradiation.
Assessment of corneal stromal depth and keratocyte apoptosis.
Confocal microscopy to evaluate keratocyte repopulation post-treatment.

Main Results:

Keratocyte apoptosis observed in the anterior stroma (approx. 300 microns) with a clear demarcation line.
Keratocyte repopulation evident within 1 month, achieving pre-operative levels by 6 months.
Demonstrated efficacy in stabilizing corneal ectasias and treating infectious ulcers.

Conclusions:

Corneal collagen cross-linking (CXL) is a proven method for strengthening corneal tissue.
The procedure is indicated for progressive corneal ectasias and certain infectious ulcers.
Maintaining corneal thickness is crucial to avoid endothelial cytotoxicity.