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Related Experiment Video

Updated: Jun 12, 2026

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
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Ribosylurea accumulates in yeast urc4 mutants.

O Björnberg1, M Vodnala, V Domkin

  • 1Department of Cell and Organism Biology, Lund University, Lund, Sweden.

Nucleosides, Nucleotides & Nucleic Acids
|June 15, 2010
PubMed
Summary
This summary is machine-generated.

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Yeast mutants unable to grow on uracil biotransformed it into ribosylurea. This indicates uracil ring opening occurs after attachment to ribose in pyrimidine degradation.

Area of Science:

  • Biochemistry
  • Microbiology
  • Yeast Metabolism

Background:

  • Pyrimidine degradation is crucial for cellular processes.
  • The uracil salvage pathway in yeast is not fully understood.
  • URC genes are involved in uracil metabolism.

Purpose of the Study:

  • To investigate the uracil degradation pathway in Saccharomyces (Lachancea) kluyveri.
  • To identify metabolic intermediates in uracil biotransformation.
  • To elucidate the role of ribose attachment in uracil ring opening.

Main Methods:

  • Utilized uracil auxotrophic yeast mutants (urc4).
  • Employed radiolabeled uracil ((14)C(2)-uracil) for biotransformation studies.
  • Analyzed biotransformation products using high-performance liquid chromatography (HPLC).

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  • Performed organic synthesis of ribosylurea for comparison.

    • Main Results:

    • Uracil auxotrophic yeast mutants produced two labeled compounds from (14)C(2)-uracil.
    • These compounds were identified as products of ribosylurea.
    • Ribosylurea was previously unreported in cellular metabolism.
    • The findings suggest ring opening occurs when uracil is attached to ribose.

    Conclusions:

    • The uracil ring opening in the URC pyrimidine degradation pathway is ribose-dependent.
    • Ribosylurea is a novel intermediate in yeast metabolism.
    • The observed compounds represent an equilibrium of pyranosyl and furanosyl forms of ribosylurea.