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The Thyroid Gland

The Thyroid Gland

The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...

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Substernal Thyroid Biopsy Using Endobronchial Ultrasound-guided Transbronchial Needle Aspiration

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Published on: November 10, 2014

UbcH10 expression on thyroid fine-needle aspirates.

Eliana Guerriero¹, Angelo Ferraro, Doriana Desiderio

¹Department of Biomorphology Sciences, Faculty of Medicine and Surgery, University of Naples Federico II, Naples, Italy.

Cancer Cytopathology

|June 15, 2010

Summary

Quantitative RT-PCR analysis of UbcH10 mRNA shows promise in diagnosing thyroid fine-needle aspiration (FNA) samples. This method, unlike immunohistochemistry, can help differentiate benign from malignant thyroid lesions.

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Area of Science:

Molecular diagnostics
Thyroid pathology
Cancer biomarker research

Background:

Thyroid fine-needle aspiration (FNA) diagnosis faces challenges with follicular neoplasm/suspicious for malignancy classifications.
UbcH10 has emerged as a potential marker for various neoplasms, including thyroid cancer.

Purpose of the Study:

To evaluate the diagnostic utility of UbcH10 expression in thyroid FNAs using quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry.
To compare UbcH10's diagnostic accuracy with existing markers and gene assays.

Main Methods:

Analysis of 84 thyroid FNAs with histological follow-up (30 malignant).
UbcH10 and Ki-67 expression assessed by immunohistochemistry.
UbcH10, CCND2, and PCSK2 mRNA levels quantified using RT-PCR.
Diagnostic accuracy determined by receiver operating characteristic (ROC) curve analysis (Area Under the Curve - AUC).

Main Results:

Immunohistochemistry for UbcH10 showed limited diagnostic value due to sporadic staining and a low cutoff value.
Quantitative RT-PCR revealed significantly higher UbcH10 mRNA levels in malignant thyroid FNAs compared to benign ones (P = .02).
The combination of UbcH10 and CCND2 mRNA achieved an AUC of 0.84, indicating strong diagnostic performance.

Conclusions:

Quantitative RT-PCR analysis of UbcH10 mRNA is a valuable tool for improving malignancy detection in thyroid FNAs.
UbcH10 can be incorporated into RT-PCR-based diagnostic panels for thyroid cancer.
Immunohistochemistry for UbcH10 is not recommended for routine diagnostic application in these cases.