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Updated: Jun 12, 2026

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs
Published on: June 28, 2019
Towards dynamic drug design: identification and optimization of beta-galactosidase inhibitors from a dynamic
Rémi Caraballo1, Morakot Sakulsombat, Olof Ramström
1Department of Organic Chemistry, KTH-Royal Institute of Technology, Teknikringen 30, 10044 Stockholm, Sweden.
Abstract:
A discovery strategy relying on the identification of fragments through resolution of a constitutional dynamic system, coupled to subsequent static ligand design and optimization, is demonstrated. The strategic design and synthesis of the best molecular fragments identified from a dynamic hemithioacetal system into static ligand structures yielded a range of beta-galactosidase inhibitors. Two series of structures mimicking the hemithioacetal motif were envisaged: thioglycosides and C-glycosides. Inhibition studies provided important structural information for the two groups, and 1-thiobenzyl-beta-D-galactopyranoside demonstrated the best inhibitory effects.
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