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Updated: Jun 12, 2026

A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats
Published on: August 19, 2018
[Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography]
Zan Liu1, Guixin Chou, Zhengtao Wang
1China Pharmaceutical University, Nanjing 210038, China. lzan2000@163.com
Objective:
To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.
Method:
The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.
Result:
The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.
Conclusion:
The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.
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