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[RRLC-UV-MS fingerprint of Radix Astragali]
Xian Su1, Lijun Wu, Pengfei Tu
1Department of Traditional Chinese Medicine & Pharmacognosy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Objective:
To establish a method for detecting the fingerprint of Radix Astragali by RRLC-UV-MS.
Method:
Separation was performed on an Agilent Zorbax Extend C18 column (2.1 mm x 100 mm, 1.8 microm). Gradient elution was performed by the mobile phases consisting of acetonitrile and 0.1% formic acid with the flow rate of 0.3 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was 30 degrees C.
Result:
The method of fingerprint analysis on Radix Astragali by RRLC-UV-MS was established. Eleven samples of Radix Astragali were analyzed, the similarities were over 0.92, and seven peaks in the fingerprint were designated.
Conclusion:
The method can be applied to the quality control and studies on chemical constituents of Radix Astragali.
