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Related Concept Videos

Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
Autoregulation of Blood Flow01:17

Autoregulation of Blood Flow

Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Disorders of Hemostasis01:24

Disorders of Hemostasis

Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.

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Related Experiment Video

Updated: Jun 12, 2026

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

Stored red-blood-cells inhibit platelet function under physiologic flow.

A Morrison1, L McMillan, V S Hornsey

  • 1Components R&D group, National Science Laboratory, Scottish National Blood Transfusion Service, Edinburgh, UK. alex.morrison@nhs.net

Vox Sanguinis
|June 16, 2010
PubMed
Summary
This summary is machine-generated.

The age of stored red blood cells (RBCs) significantly impacts platelet function assays. Using RBCs stored for less than 21 days is recommended for accurate platelet adhesion and aggregation testing.

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Microfluidics in Assessing Platelet Function
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Microfluidics in Assessing Platelet Function

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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Related Experiment Videos

Last Updated: Jun 12, 2026

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

Microfluidics in Assessing Platelet Function
06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Area of Science:

  • Hematology
  • Transfusion Medicine
  • Biomedical Engineering

Background:

  • The DiaMed Impact R assay evaluates platelet function under physiological flow conditions.
  • This assay typically uses whole blood but can be adapted for stored platelet concentrates by adding compatible red blood cells (RBCs).
  • Previous studies have used RBCs stored for up to 14 days.

Purpose of the Study:

  • To investigate the impact of extended red blood cell (RBC) storage duration (up to 60 days) on platelet function assays.
  • To determine if RBC age affects platelet adhesion and aggregation in the DiaMed Impact R system.

Main Methods:

  • Platelet concentrates were assayed with RBCs stored for 2 to 60 days.
  • Red blood cells (RBCs) were separated into 'young' and 'old' populations using centrifugation to isolate age-related effects.
  • Platelet adhesion (SC) and aggregation (AS) were measured using the DiaMed Impact R assay.

Main Results:

  • A statistically significant difference in supporting platelet adhesion and aggregation was observed between RBCs stored for ≤20 days and those stored for 21-60 days (P<0.01).
  • The storage duration had a more pronounced effect on RBC function than the age difference between 'young' and 'old' RBC populations within stored units.
  • 'Young' RBCs from stored units demonstrated poorer support for platelet function compared to 'young' RBCs from fresh units.

Conclusions:

  • Red blood cells (RBCs) should ideally be stored for less than 21 days for use in this platelet function assay.
  • The DiaMed Impact R assay may potentially serve as a tool for assessing red blood cell (RBC) functionality.