Related Experiment Video
Updated: Jun 12, 2026
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Published on: December 8, 2023
Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity
Yu-Ching Su1, Kiew-Lian Wan, Rahmah Mohamed
1School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia.
Abstract:
Burkholderia pseudomallei is resistant to a wide range of antibiotics, leading to relapse and recrudescence of melioidosis after cessation of antibiotic therapy. More effective immunotherapies are needed for better management of melioidosis. We evaluated the prophylactic potential of the immunogenic outer membrane protein Omp85 as a vaccine against murine melioidosis. Immunization of BALB/c mice with recombinant Omp85 (rOmp85) triggered a Th2-type immune response. Up to 70% of the immunized animals were protected against infectious challenge of B. pseudomallei with reduced bacterial load in extrapulmonary organs. Mouse anti-rOmp85 promoted complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear cells. In conclusion, we demonstrated that B. pseudomallei Omp85 is potentially able to induce protective immunity against melioidosis.
Insights
This study shows that the outer membrane protein Omp85 from Burkholderia pseudomallei can be a potential vaccine candidate. Immunization with Omp85 protected mice against melioidosis by enhancing immune responses.
Area of Science:
- Immunology
- Microbiology
- Vaccine Development
Background:
- Melioidosis, caused by Burkholderia pseudomallei, is challenging to treat due to antibiotic resistance.
- Relapse and recrudescence are common issues after antibiotic therapy for melioidosis.
- There is a critical need for effective immunotherapies to manage melioidosis.
Purpose of the Study:
- To evaluate the prophylactic potential of Burkholderia pseudomallei outer membrane protein Omp85 as a vaccine against melioidosis.
- To investigate the immune response induced by Omp85 immunization.
- To assess the protective efficacy of Omp85 against B. pseudomallei infection in a murine model.
Main Methods:
- Immunization of BALB/c mice with recombinant Omp85 (rOmp85).
- Assessment of the type of immune response (Th2-type).
- Evaluation of protection against infectious challenge with B. pseudomallei, including bacterial load reduction in organs.
- In vitro studies on the effect of anti-rOmp85 antibodies on bacterial killing (complement-mediated killing and opsonophagocytosis).
Main Results:
- Immunization with rOmp85 induced a Th2-type immune response in mice.
- Up to 70% of immunized mice showed protection against B. pseudomallei challenge.
- Reduced bacterial load was observed in extrapulmonary organs of protected mice.
- Mouse anti-rOmp85 antibodies facilitated complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear cells.
Conclusions:
- Burkholderia pseudomallei Omp85 is an immunogenic protein with potential as a vaccine candidate.
- Omp85 immunization can induce protective immunity against melioidosis in a murine model.
- The protective mechanism involves both cellular and humoral immune responses, including antibody-mediated bacterial clearance.
More Related Videos
12:53Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
Published on: March 16, 2022
06:32Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
Published on: September 19, 2016
