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Related Concept Videos

Antigen Processing Pathways01:31

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 12, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Technologies for MHC class I immunoproteomics.

Rieuwert Hoppes1, Reggy Ekkebus, Ton N M Schumacher

  • 1Division of Cell Biology, Netherlands Cancer Institute and Netherlands Proteomics Centre, The Netherlands.

Journal of Proteomics
|June 16, 2010
PubMed
Summary
This summary is machine-generated.

Identifying T cell epitopes, which are peptides recognized by T lymphocytes, is crucial for diagnostics and immunotherapy. This review covers MHC class I epitope definition techniques and recent advancements in epitope discovery.

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Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

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Related Experiment Videos

Last Updated: Jun 12, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Area of Science:

  • Immunology
  • Molecular Biology
  • Proteomics

Background:

  • T cell epitopes are peptides presented by MHC molecules on cell surfaces.
  • These epitopes are recognized by T lymphocytes, playing a key role in immune responses.
  • Understanding T cell epitopes is vital for diagnostics and developing immunotherapies.

Purpose of the Study:

  • To review key techniques for defining MHC class I T cell epitopes.
  • To discuss recent developments and implications in T cell epitope discovery.
  • To highlight the path towards defining the MHC-associated peptidome.

Main Methods:

  • Review of established and emerging techniques for T cell epitope identification.
  • Analysis of recent advancements in epitope discovery strategies.
  • Discussion of the implications of these developments for immunological research.

Main Results:

  • Key techniques for MHC class I epitope definition are presented.
  • Recent progress in epitope discovery strategies and their impact are discussed.
  • The review outlines the trajectory towards comprehensive MHC-associated peptidome definition.

Conclusions:

  • Advances in epitope discovery are enhancing our ability to identify T cell epitopes.
  • This knowledge is essential for improving diagnostic tools and therapeutic strategies.
  • The ultimate goal is the complete characterization of the MHC-associated peptidome.