Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tangential flow filtration for isolating exomeres and other nanoscale extracellular particles.

Nanoscale·2026
Same author

Generation of Cellular Biofactories for the Scalable Production of Surface-Engineered Extracellular Vesicles via CRISPR Genome Editing.

ACS biomaterials science & engineering·2026
Same author

Transcriptome analysis reveals the effect of fermented feed on the growth, antioxidant capacity, and immune function in juvenile Eriocheir sinensis.

Comparative biochemistry and physiology. Part D, Genomics & proteomics·2026
Same author

10 years trends and hospitalization outcomes of non-neonatal tetanus: a large-scale multicenter retrospective study in China.

Critical care (London, England)·2026
Same author

Endoplasmic reticulum stress induced autophagy alters cellular processing of cationic lipid delivered siRNAs.

Drug delivery and translational research·2026
Same author

A scalable filtration-based method for isolating exomeres and other nanoscale extracellular particles.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 12, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Development of a dual-aptamer-based multiplex protein biosensor.

Shengnan Xie1, S Patrick Walton

  • 1Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824-1226, USA.

Biosensors & Bioelectronics
|June 16, 2010
PubMed
Summary

This study introduces a novel aptamer-based biosensor for simultaneous protein detection. The developed method enables sensitive and specific quantification of thrombin and platelet-derived growth factor-BB (PDGF-BB) in various samples.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Parallel protein biosensors are crucial for understanding complex biological systems and improving clinical diagnostics.
  • Aptamers offer advantages in sensitivity and flexibility for affinity-based biosensing applications.

Purpose of the Study:

  • To develop and characterize an aptamer-based method for parallel protein analysis.
  • To achieve simultaneous and quantitative detection of multiple proteins using unique aptamers targeting distinct epitopes.

Main Methods:

  • Development of an aptamer-based biosensing strategy utilizing two distinct aptamers per target protein.
  • Characterization of the biosensor's performance in detecting thrombin and platelet-derived growth factor-BB (PDGF-BB).

Main Results:

  • Simultaneous and quantitative detection of thrombin and PDGF-BB was achieved.
  • The method demonstrated high specificity in both buffered solutions and complex biological samples like serum.
  • The use of two unique aptamers targeting different epitopes enhanced detection capabilities.

Conclusions:

  • The developed aptamer-based parallel biosensor provides a sensitive and specific platform for simultaneous protein quantification.
  • This technique offers a valuable tool for advancing protein analysis in research and clinical diagnostics.