Lethal ccdB gene-based zero-background vector for construction of shotgun libraries
Related Experiment Video
Updated: Jun 12, 2026

11:37
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Published on: February 26, 2019
1Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan. miyazaki-kentaro@aist.go.jp
Journal of Bioscience and Bioengineering
|June 16, 2010
Abstract:
A lethal ccdB gene-based vector was developed for the construction of shotgun libraries in Escherichia coli. The mutated ccdB gene carries multiple cloning sites and the amber stop codon, which simultaneously allows for maintenance of the intact vector in supE(-) strains and removal of uncut and self-ligated plasmids in supE(+) strains.

