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Updated: Jun 12, 2026

Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Crescenteric amniotic membrane grafting in keratoprosthesis-associated corneal melt
Evelyn Tay1, Canan Asli Utine, Esen K Akpek
1Ocular Surface Diseases and Dry Eye Clinic, The Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287-9238, USA.
Abstract:
We herein present a surgical technique for the management of corneal melt following Boston type I keratoprosthesis implantation. The technique was performed using new-generation dehydrated human amniotic membrane. The membrane was fashioned in the shape of 2 crescentic patches using disposable corneal trephines. A double layer of grafts was then sutured into the host bed with interrupted 10-0 nylon sutures. Rapid healing with corneal epithelization and vascularization was noted within days. The main advantage of the amniotic membrane that we used is that it is thicker than the older generation (110 vs 40 microm) and suitable for tectonic purposes. It is also readily available with a long shelf life and does not require a lamellar dissection unlike a donor corneal patch. This surgical technique may potentially serve as another tool in a cornea surgeon's armamentarium.

