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Updated: Jun 12, 2026
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Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
Published on: September 10, 2013
Colloidal DNA carriers for direct localization in cell compartments by pH sensoring
Uta Reibetanz1, Min Hui Averil Chen, Shaillender Mutukumaraswamy
1School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore. ureibetanz@trm.uni-leipzig.de
Biomacromolecules
|June 17, 2010
Summary
Researchers developed multifunctional microparticles for simultaneous drug delivery and sensing. These particles track their location within cells, enabling real-time monitoring of active agent release and transport for improved nanocarrier system design.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cell Biology
Background:
- Multifunctional microparticles integrate delivery and sensing capabilities.
- Calcium carbonate microparticles offer a versatile platform for functionalization.
Purpose of the Study:
- To develop and characterize multifunctional microparticles for simultaneous delivery and sensing.
- To investigate cellular uptake, localization, and processing of these particles.
- To establish a correlation between particle localization and reporter gene expression.
Main Methods:
- Functionalization of calcium carbonate particles with FITC-PAH for pH-dependent localization.
- Integration of plasmid DNA and RITC-protamine into PRM/DXS multilayers.
- Cellular uptake and processing analysis using flow cytometry and confocal laser scanning microscopy in HEK293T/17 cells.
Main Results:
- FITC-labeled core fluorescence intensity correlated with particle localization within cellular compartments.
- Successful co-delivery of plasmid DNA and protamine.
- Demonstrated simultaneous monitoring of particle location and reporter protein expression without additional cell staining.
Conclusions:
- The designed microparticles enable simultaneous tracking of particle location and active agent release.
- This approach provides a valuable tool for studying and designing nano- and microcarrier systems.
- The system facilitates real-time monitoring of transport and release dynamics.
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