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Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Updated: Jun 12, 2026

A New Workflow for Sampling and Digitizing Increment Cores

A New Workflow for Sampling and Digitizing Increment Cores

Published on: September 27, 2024

Self-digitization of sample volumes.

Dawn E Cohen¹, Thomas Schneider, Michelle Wang

¹Department of Chemistry, University of Washington, Seattle, Washington 98195-1700, USA.

Analytical Chemistry

|June 17, 2010

Summary

This summary is machine-generated.

This study presents a simple microfluidic device for precise sample digitization into discrete volumes without sample loss. This robust method enables customized sample volumes for diverse applications like digital PCR and single-cell analysis.

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Miniaturized Sample Preparation for Transmission Electron Microscopy

Miniaturized Sample Preparation for Transmission Electron Microscopy

Published on: July 27, 2018

Area of Science:

Microfluidics
Biotechnology
Analytical Chemistry

Background:

Traditional sample partitioning methods often suffer from sample loss and lack precise volume control.
Digitizing samples into discrete volumes is crucial for high-throughput analyses and sensitive assays.

Purpose of the Study:

To develop a simple, robust microfluidic device for self-digitizing aqueous samples into an array of discrete volumes.
To characterize the fluidic parameters governing the self-digitization process.
To demonstrate the utility of this method in applications such as polymorph separation and downstream analysis.

Main Methods:

Utilized an inherent fluidic phenomenon where an aqueous sample is divided into chambers primed with an immiscible phase.
Employed experiments and simulations to characterize fluidic forces, interfacial tension, and channel geometry.
Investigated the self-digitization process and the factors influencing sample volume and stability.

Main Results:

Achieved 100% sample digitization into a localized array with no volume loss.
Demonstrated that the final sample volume is precisely controlled by chamber geometry.
Successfully separated crystal polymorphs and showed the potential for downstream reagent addition and analysis.

Conclusions:

The developed microfluidic device offers a simple and effective method for sample digitization.
This technology enables precise control over sample volumes, facilitating diverse applications.
The method is highly versatile for applications requiring large arrays of discrete samples, including digital PCR, single-cell analysis, and drug screening.