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Updated: Jun 12, 2026

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
Published on: February 27, 2012
Design and evaluation of genome-wide libraries for RNA interference screens
Thomas Horn1, Thomas Sandmann, Michael Boutros
1German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and University of Heidelberg, Department of Cell and Molecular Biology, Faculty of Medicine Mannheim, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany.
Abstract:
RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/.
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