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Localization of human tryptophan hydroxylase (TPH) to chromosome 11p15.3----p14 by in situ hybridization

S P Craig1, S Boularand, M C Darmon

  • 1Biochemistry Department, University of Oxford UK.

Cytogenetics and Cell Genetics
|January 1, 1991
PubMed

Abstract:

The human gene for tryptophan hydroxylase has been previously assigned to chromosome 11 by analysis of a panel of somatic cell hybrids. We report here on the refinement of this localization by in situ hybridization.

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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