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Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
Sex-specific gene expression in the BXD mouse liver
Daniel M Gatti1, Ni Zhao, Elissa J Chesler
1Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Physiological Genomics
|June 17, 2010
Summary
Genetic variation influences sex-specific liver gene expression, revealing differences in gene networks between male and female mice. These findings offer insights into sex differences in xenobiotic metabolism and responses across species.
Area of Science:
- Genomics
- Molecular Biology
- Comparative Biology
Background:
- Sex-based differences in clinical phenotypes are linked to differential gene expression.
- Genetic variation and environmental factors interact to influence gene transcription.
Purpose of the Study:
- To investigate how genetic variation impacts sex-related differences in liver gene expression.
- To identify genetic loci regulating sex-specific liver gene expression.
- To compare sex differences in mouse and human liver gene expression.
Main Methods:
- Genome-wide liver mRNA expression analysis in male and female mice from defined genetic strains (C57BL/6J, DBA/2J, B6D2F1, and BXD recombinant inbred strains).
- Gene expression quantitative trait locus (eQTL) mapping.
- Comparative analysis with human liver gene expression data.
Main Results:
- Sexually dimorphic expression was observed for many genes involved in xenobiotic metabolism and other pathways.
- Significant eQTL loci regulating multiple genes were largely sex-independent.
- Gene expression networks showed substantial differences in correlation between sexes.
- Similarities in sexually dimorphic liver gene expression were found between mice and humans.
Conclusions:
- Genetic variation plays a role in sex differences in liver gene expression, particularly in network correlations.
- Understanding these sex-specific molecular underpinnings is crucial for predicting responses to xenobiotics.
- This study provides a genome-level comparison of sex differences in liver gene expression across species.
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