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Updated: Jun 12, 2026
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Published on: May 4, 2012
A biotin label-based antibody array for high-content profiling of protein expression
Ruochun Huang1, Weidong Jiang, Jean Yang
1RayBiotech, Inc., Norcross, GA 30092, USA.
Cancer Genomics & Proteomics
|June 17, 2010
Summary
A new biotin label-based antibody array enables high-content protein profiling for biomarker discovery. This reliable and cost-effective method can simultaneously detect hundreds of proteins, aiding in identifying disease biomarkers like those for ovarian cancer.
Area of Science:
- Biotechnology
- Proteomics
- Biomarker Discovery
Background:
- Global protein profiling is crucial for biomedical research, drug development, and biomarker discovery.
- Existing antibody array systems lack reliability and high-content capabilities for analyzing numerous biological samples.
- A need exists for a sensitive, specific, user-friendly, and cost-effective protein profiling method.
Purpose of the Study:
- To develop a reliable, easy-to-use, and cost-effective high-content antibody array system.
- To profile protein expression levels with high sensitivity and specificity.
- To uncover potential biomarkers for ovarian cancer using the developed antibody array technology.
Main Methods:
- Developed a high-density antibody array utilizing biotin labeling for biological samples.
- Detected biotinylated proteins using streptavidin-conjugated fluorescent dye (Cy3 equivalent) reporter.
- Normalized signals via laser scanning with positive, negative, and internal controls.
Main Results:
- Simultaneously detected 507 human, 308 mouse, and 90 rat proteins at pg/ml to ng/ml levels with <20% variation.
- Screened 507 proteins in ovarian cancer patients and healthy individuals using human biotin-based antibody arrays.
- Identified a panel of proteins with significant expression differences (p<0.05) distinguishing ovarian cancer patients from controls.
Conclusions:
- The developed biotin label-based antibody arrays demonstrate significant potential for biomarker discovery applications.
- The system offers a reliable and high-content approach for protein expression profiling.
- This technology can aid in identifying novel biomarkers for diseases such as ovarian cancer.
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