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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
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Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

Emergence of Proteus mirabilis isolates producing TEM-52 extended-spectrum beta-lactamases in Croatia

Sanda Sardelić1, Branka Bedenić, Dubravko Sijak

  • 1Department of Microbiology, Clinical Hospital Center Split, Split, Croatia. sandasard@gmail.com

Chemotherapy
|June 17, 2010
PubMed
Abstract

Insights

Extended-spectrum beta-lactamase (ESBL)-producing Proteus mirabilis strains emerged in Croatia, identified as TEM-52. These ESBL-producing Proteus mirabilis strains showed clonal relatedness and carried resistance genes on plasmids.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • Increased detection of extended-spectrum beta-lactamase (ESBL)-producing Proteus mirabilis in Croatia.
  • Need for molecular characterization of ESBLs in P. mirabilis isolates.

Purpose of the Study:

  • To perform molecular characterization of ESBLs in P. mirabilis isolates from a Croatian hospital.
  • To investigate the genetic basis of antimicrobial resistance in these strains.

Main Methods:

  • Antimicrobial susceptibility testing using broth microdilution.
  • Characterization of ESBLs and quinolone resistance genes via PCR and sequencing.
  • Strain genotyping using pulsed-field gel electrophoresis (PFGE).

Main Results:

  • All seven P. mirabilis strains produced ESBLs and exhibited resistance to multiple antibiotics but remained susceptible to certain beta-lactam/beta-lactamase inhibitor combinations and carbapenems.
  • PCR sequencing identified the TEM-52 beta-lactamase gene in all strains.
  • PFGE revealed clonal relatedness among the TEM-52-producing P. mirabilis strains.
  • The bla(TEM-52) gene was located on a 70-kb conjugative plasmid in three isolates.

Conclusions:

  • The TEM-52 enzyme has emerged among P. mirabilis isolates in Croatia.
  • The findings highlight the clonal spread of TEM-52-producing P. mirabilis within the hospital setting.

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