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Related Experiment Videos

Decidual-type prolactin expression by the human myometrium.

B Gellersen1, A Bonhoff, N Hunt

  • 1Institute for Hormone and Fertility Research, Hamburg, Germany.

Endocrinology
|July 1, 1991
PubMed
Summary

The human myometrium expresses the human prolactin (hPRL) gene, producing a form of hPRL distinct from pituitary hPRL. Progestin significantly inhibits myometrial hPRL expression, suggesting a regulatory role.

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Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Molecular Genetics

Background:

  • The human myometrium has been proposed as a potential source of uterine prolactin (PRL).
  • Immunoreactive PRL has been detected in myometrial explant culture supernatants.
  • The precise nature and regulation of PRL production in the myometrium remain to be fully elucidated.

Purpose of the Study:

  • To investigate the expression and characteristics of human prolactin (hPRL) in the human myometrium.
  • To determine if the myometrium synthesizes PRL and to characterize its transcript and protein.
  • To examine the regulation of myometrial hPRL expression by hormones like progestin and estrogen.

Main Methods:

  • Northern blot hybridization to detect hPRL mRNA in myometrium.

Related Experiment Videos

  • Sequence and primer extension analyses to characterize the hPRL transcript.
  • Myometrial explant cultures to assess hPRL secretion and regulation.
  • Nb2 lymphoma bioassay to determine lactogenic activity.
  • Monoclonal antibody neutralization assays.

    • Main Results:

    • The human (h) PRL gene is expressed in the myometrium in vivo, with a transcript larger than pituitary hPRL mRNA.
    • Myometrial PRL is identical to pituitary hPRL, but the mRNA originates from alternative transcription initiation.
    • Myometrial explants secrete hPRL, including a glycosylated variant (G-hPRL), with significant lactogenic activity.
    • hPRL secretion and mRNA abundance are markedly reduced by progestin (medroxy-progesterone acetate) but unaffected by estrogen.

    Conclusions:

    • The human myometrium is a source of hPRL, utilizing an alternative mRNA transcript.
    • Myometrial hPRL production is regulated by progestin, indicating a potential role in uterine function.
    • These findings expand our understanding of PRL's extrapituitary sources and regulation within the uterus.