Aspartate aminotransferase is involved in cold adaptation in psychrophilic Pseudomonas syringae
V R Sundareswaran1, Ashish Kumar Singh, Smita Dube
1Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500007, India.
Abstract:
CSM2, a cold-sensitive mutant of psychrophilic Pseudomonas syringae, grows like wild-type cells when cultured at 22 and 28 degrees C; but at 4 degrees C, the growth is retarded. In CSM2, AAT (coding for aspartate aminotransferase) is identified as the mutated gene. The expression of AAT in Pseudomonas syringae was transiently enhanced when cells were shifted from 22 to 4 degrees C indicating that AAT is cold-inducible. Complementation of the mutated AAT transformed CSM2 from a cold-sensitive phenotype to a cold-resistant phenotype like the wild-type cells, thus providing evidence for the first time that AAT is required for low-temperature growth.
Related Concept Videos
Responses to Heat and Cold Stress
Other Stress Responses in Bacteria
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Factors Influencing Microbial Growth: Temperature
Diversity of Archaea IV
Diversity of Archaea III


