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Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
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Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

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USER cloning and USER fusion: the ideal cloning techniques for small and big laboratories

Hussam H Nour-Eldin1, Fernando Geu-Flores, Barbara A Halkier

  • 1Department of Plant Biology and Biotechnology, and VKR Centre Pro-Active Plants, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|June 17, 2010
PubMed

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The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
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