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Updated: Jun 12, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Isolation and synthesis of flavonols and comparison of their antioxidant activity
Aurangzeb Hasan1, A Sadiq, A Abbas
1Department of Chemistry, Quaid-i-Azam University, Islamabad, Pakistan. hassaan2@super.net.pk
Abstract:
Phytochemical investigation of the leaves of Astragalus beckari yielded four flavonol aglycones, namely kaempferol, quercetin, 5-deoxy kaempferol and fisitin. These isolated compounds were then synthesised in the laboratory using the Algar-Flyn-Oyamad reaction. Antioxidant activity of both the isolated and synthesised flavonoids was compared using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay method. The isolated flavonoids were found to be more active.
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