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Parasexuality in Race 65 Colletotrichum lindemuthianum isolates
Lúcia J Rosada1, Claudinéia C S Franco, Juliane R Sant'Anna
1Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
Abstract:
Heterokaryosis is the initial step of the parasexual cycle, a process that provides genetic variability in filamentous fungi through the production of heterozygous diploid nuclei. To characterize the parasexual cycle in Colletotrichum lindemuthianum, we evaluated the presence of heterokaryosis, vegetative compatibility reactions, and diploid formation among isolates of Race 65 collected from different Brazilian states. Vegetative compatibility groups were identified among the isolates according to their ability to form heterokaryons. Two heterozygous diploids were selected from compatible heterokaryons, which were characterized by the segregation of the parental auxotrophic markers and by RAPD profiles.
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