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Updated: Jun 12, 2026
Rat Mesentery Angiogenesis Assay
Published on: June 18, 2011
Anti-angiogenic activity of methanol extract of Phellinus linteus and its fractions
Yeon Sil Lee1, Yoon Hee Kim, Eun Kyung Shin
1Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University, 39 Hallym daehakgil, Chuncheon 200-702, Republic of Korea.
Aim Of The Study:
The aim of the present study was to investigate the effects of MeOH extract of PL (PLME) and its fractions on angiogenesis.
Materials And Methods:
PLME and its subsequent fractions (methylene chloride, ethyl acetate, n-butanol and aqueous fractions) were evaluated in vitro. Specifically, the anti-angiogenic activities of PLME and its fractions were investigated by measuring their effects on the proliferation, migration, tube formation and phosphorylation of vascular endothelial growth factor receptor (VEGFR)-2 in human umbilical vein endothelial cells (HUVECs). In addition, the in vivo Matrigel plug model was applied to evaluate new vessel formation.
Results:
The results revealed that PLME and its subsequent fractions, except for the aqueous fraction, led to significant inhibition of the proliferation, migration, tube formation and VEGFR-2 phosphorylation of HUVECs as well as in vivo angiogenesis.
Conclusions:
These findings indicate the potential for the use of PLME in pathological situations involving stimulated angiogenesis, such as inflammation and tumor development.
Insights
The MeOH extract of PL (PLME) and its fractions effectively inhibited angiogenesis by reducing cell proliferation, migration, and tube formation. These findings suggest PLME
Area of Science:
- Pharmacology
- Cell Biology
- Biochemistry
Background:
- Angiogenesis is crucial for physiological processes but also implicated in pathological conditions like inflammation and tumor growth.
- Vascular Endothelial Growth Factor Receptor (VEGFR)-2 signaling plays a key role in regulating endothelial cell functions during angiogenesis.
Purpose of the Study:
- To investigate the anti-angiogenic potential of the methanol extract of *P. lentiscus* (PLME) and its derived fractions.
- To evaluate the effects of PLME and its fractions on key cellular processes involved in angiogenesis.
Main Methods:
- In vitro assessment of PLME and fractions on human umbilical vein endothelial cell (HUVEC) proliferation, migration, and tube formation.
- Analysis of VEGFR-2 phosphorylation in HUVECs treated with PLME and fractions.
- In vivo evaluation of anti-angiogenic activity using a Matrigel plug assay.
Main Results:
- PLME and its methylene chloride, ethyl acetate, and n-butanol fractions significantly inhibited HUVEC proliferation, migration, and tube formation.
- These active fractions also demonstrated a reduction in VEGFR-2 phosphorylation.
- In vivo studies confirmed the anti-angiogenic effect of PLME and its fractions in the Matrigel plug model.
Conclusions:
- PLME possesses significant anti-angiogenic properties, mediated through the inhibition of endothelial cell proliferation, migration, and VEGFR-2 signaling.
- The findings highlight the therapeutic potential of PLME for managing diseases characterized by excessive angiogenesis, such as inflammation and cancer.