Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AXIN1 and AXIN2 regulate the WNT-signaling landscape to promote distinct mesoderm programs.

Developmental cell·2026
Same author

L1CAM signaling through planar cell polarity drives SOX2 expression and lung adenocarcinoma metastasis.

Nature communications·2026
Same author

Asymmetric attrition and secondary chromosome destabilization after double-strand breaks in human embryonic development.

Nature communications·2026
Same author

Upwelling periodically disturbs the ecological assembly of microbial communities in Lake Ontario.

ISME communications·2026
Same author

CORR Insights®: Preliminary Surgical Findings and Complications After National Centralization of Pediatric Bone Sarcoma Resections in the Netherlands: a Benchmarking Study.

Clinical orthopaedics and related research·2026
Same author

Mutational scanning reveals oncogenic CTNNB1 mutations have diverse effects on signaling.

Nature genetics·2026

Related Experiment Video

Updated: Jun 12, 2026

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

Oriented cell motility and division underlie early limb bud morphogenesis.

Laurie A Wyngaarden1, Kevin M Vogeli, Brian G Ciruna

  • 1Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, M5G 1X8, Canada.

Development (Cambridge, England)
|June 18, 2010
PubMed
Summary

Vertebrate limb bud outgrowth involves directional mesoderm movement from the lateral plate. Wnt5a signaling establishes cell polarity, guiding oriented cell behaviors essential for limb development.

More Related Videos

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
08:08

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Published on: January 12, 2022

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
07:52

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Published on: November 26, 2019

Related Experiment Videos

Last Updated: Jun 12, 2026

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation
08:08

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Published on: January 12, 2022

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads
07:52

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Published on: November 26, 2019

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Morphogenesis

Background:

  • Vertebrate limb bud development originates from lateral plate mesoderm and ectoderm.
  • Genetic factors are known, but early morphogenetic mechanisms driving limb outgrowth are unclear.

Purpose of the Study:

  • To elucidate the morphogenetic mechanisms behind early vertebrate limb bud outgrowth.
  • To investigate the role of cell movement and signaling in limb bud formation.

Main Methods:

  • Live imaging and lineage tracing in vertebrate models.
  • Analysis of cell movement direction, cell axes, and cell division plane orientation.
  • Investigating the function of Wnt5a signaling.

Main Results:

  • Lateral plate mesoderm contributes to the limb bud via directional cell movement.
  • Cellular parameters shift from rostrocaudal to mediolateral orientation during outgrowth.
  • Wnt5a acts as a chemoattractant, establishing cell polarity and oriented cell behaviors.

Conclusions:

  • Directional cell movement and orientation changes are crucial for early limb bud outgrowth.
  • Wnt5a signaling plays a key role in orchestrating cell polarity and directed cell behaviors during limb development.