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Mapping catalytically important regions of an enzyme using two-codon insertion mutagenesis: a case study correlating
1Department of Microbiology, Hearst Microbiology Research Center, Cornell University Medical College, New York, NY 10021.
Gene
|April 1, 1991
Abstract:
Two-codon insertion mutants throughout the beta-lactamase (Bla)-encoding gene were characterized. Second site revertants of various mutants were isolated, mapped and sequenced. The activity of the mutants and the ability to get revertants showed a positive correlation with increasing distance from the active site, based on the three-dimensional structure of Bla. This observation is discussed as it may pertain to the generalized use of two-codon insertion mutagenesis in mapping important catalytic regions in enzymes.