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Updated: Jun 12, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
PTEN regulates collagen-induced platelet activation
Zhen Weng1, Ding Li, Lin Zhang
1Department of Biochemistry and Molecular & Cell Biology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
PTEN deficiency increases platelet count and collagen-induced activation via PI3K-dependent and -independent pathways. This research reveals PTEN
Area of Science:
- Molecular Biology
- Hematology
- Cell Signaling
Background:
- Phosphatidylinositol 3-kinase (PI3K) is crucial for collagen-induced platelet activation.
- PTEN is a key regulator of the PI3K/Akt signaling pathway.
- The role of PTEN in platelet function remains largely unexamined.
Purpose of the Study:
- To investigate the role of PTEN in platelet activation.
- To elucidate PTEN's involvement in the PI3K/Akt signaling pathway in platelets.
Main Methods:
- Analysis of platelet counts and bleeding times in Pten(-/-) and Pten(+/+) mice.
- Assessment of platelet activation and aggregation in response to collagen.
- Examination of Akt phosphorylation (Ser473) using Western blot.
- Pharmacological inhibition of PI3K (LY294002) and other signaling molecules.
Main Results:
- Pten(-/-) mice exhibited a 25% increase in platelet count and reduced bleeding times.
- PTEN deficiency enhanced platelet sensitivity to collagen-induced activation and aggregation.
- Collagen stimulation led to increased Akt phosphorylation at Ser473 in PTEN-deficient platelets.
- PI3K inhibition (LY294002) blocked collagen-induced aggregation in wild-type but not PTEN-deficient platelets.
Conclusions:
- PTEN plays a significant role in regulating collagen-induced platelet activation.
- PTEN influences platelet function through both PI3K/Akt-dependent and -independent signaling mechanisms.
- Targeting PTEN may offer novel therapeutic strategies for modulating platelet activity.
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