Related Experiment Video
Updated: Jun 12, 2026

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Nitrogen removal from reject water with primary sludge as denitrification carbon source
1School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, Heilongjiang Province, China. zhangliang_1986@126.com
A novel system effectively removes nitrogen from reject water using simultaneous sludge fermentation and denitrification. This process achieves over 90% total nitrogen removal efficiency without external carbon sources.
Area of Science:
- Environmental Engineering
- Wastewater Treatment
- Microbial Ecology
Background:
- Reject water poses challenges for nitrogen removal due to high ammonia concentrations.
- Conventional denitrification requires supplemental carbon sources, increasing operational costs.
- Simultaneous sludge fermentation and denitrification offer a potential solution for in-situ carbon generation.
Purpose of the Study:
- To develop and evaluate a novel system for nitrogen removal from reject water.
- To investigate the efficacy of using primary sludge as an intermittent electron donor for denitrification.
- To assess the impact of effluent recycle and operational parameters on nitrogen removal efficiency.
Main Methods:
- Implementation of a coupled anoxic/oxic reactor for nitrification and a specialized denitrification reactor.
- Intermittent addition of primary sludge as an electron donor in the denitrification reactor.
- Monitoring of key parameters including total nitrogen, volatile suspended solids, alkalinity, and oxidation-reduction potential (ORP).
Main Results:
- Achieved over 90% total nitrogen removal efficiency without external carbon addition.
- Demonstrated simultaneous sludge fermentation and denitrification, promoting both processes.
- Observed a 50% reduction in volatile suspended solids due to primary sludge degradation.
- Identified effluent recycle as beneficial for alkalinity recovery and nitrogen removal.
Conclusions:
- The novel system effectively removes nitrogen from reject water using primary sludge as an in-situ carbon source.
- Simultaneous sludge fermentation and denitrification is a viable and efficient wastewater treatment strategy.
- Oxidation-reduction potential (ORP) can serve as a reliable control parameter for primary sludge addition.
Related Concept Videos
Biological Treatment of Effluent and Waste Water
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Microbial Wastewater Treatment
Inorganic Nitrogen Assimilation
Microbes and the Nitrogen Cycle
Bioremediation

