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Related Concept Videos

Diffusion01:12

Diffusion

Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
Diffusion01:21

Diffusion

Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Passive Diffusion: Overview and Kinetics01:17

Passive Diffusion: Overview and Kinetics

Passive diffusion is a critical process that allows small lipophilic drugs to cross the cell membrane along a concentration gradient. This mechanism's efficiency depends on four primary factors: the membrane's surface area, the drug's lipid-water partition coefficient, the concentration gradient, and the membrane's thickness.
When administered orally, drugs establish a substantial concentration gradient between the gastrointestinal (GI) lumen and the bloodstream, expediting their diffusion into...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Jun 12, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Diffusion of light in turbid material.

A Ishimaru

    Applied Optics
    |June 18, 2010
    PubMed
    Summary

    This paper reviews mathematical techniques for light diffusion in turbid materials like tissues. It covers transport theory, scattering approximations, and their limitations for accurate optical modeling.

    Area of Science:

    • Biomedical Optics
    • Mathematical Modeling
    • Light Scattering

    Background:

    • Accurate modeling of light diffusion in turbid media is crucial for applications in biomedical optics and material science.
    • Existing mathematical techniques require careful consideration of their applicability and limitations.

    Purpose of the Study:

    • To provide a comprehensive overview of mathematical techniques for describing light diffusion in turbid materials.
    • To evaluate the usefulness and limitations of various light diffusion models.
    • To discuss different wave excitations and surface scattering effects.

    Main Methods:

    • Review of radiative transport theory, including radiance, radiant energy fluence rate, phase functions, and boundary conditions.
    • Discussion of first-order solutions, multiple scattering, and diffusion approximation, along with their limitations.

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    The Diffusion of Passive Tracers in Laminar Shear Flow

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    Last Updated: Jun 12, 2026

    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
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    Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

    Published on: January 9, 2017

    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
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    From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

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    The Diffusion of Passive Tracers in Laminar Shear Flow

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  • Analysis of plane wave, spherical wave, beam wave, and pulse wave excitations, and surface scattering effects.
  • Main Results:

    • The paper systematically reviews established and advanced mathematical techniques for light diffusion modeling.
    • It highlights the strengths and weaknesses of different approaches, aiding in technique selection.
    • The discussion encompasses various excitation types and the impact of surface roughness.

    Conclusions:

    • A thorough understanding of mathematical techniques is essential for accurate light diffusion analysis in turbid media.
    • The choice of technique depends on the specific application, material properties, and desired accuracy.
    • Further research may focus on refining models to account for complex scattering phenomena and interface effects.