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Updated: Jun 12, 2026

Calcium Imaging of Cortical Neurons using Fura-2 AM

Calcium Imaging of Cortical Neurons using Fura-2 AM

Published on: January 19, 2009

Fura-2FF-based calcium indicator for protein labeling.

Agostina A Ruggiu¹, Michael Bannwarth, Kai Johnsson

¹Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

Organic & Biomolecular Chemistry

|June 18, 2010

Summary

Researchers synthesized a novel Fura-2FF fluorescent indicator for calcium ions (Ca2+). This indicator can be attached to SNAP-tag fusion proteins, maintaining its calcium-sensing function after protein coupling.

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Area of Science:

Biochemistry
Molecular Biology
Fluorescence Spectroscopy

Background:

Calcium ions (Ca2+) are crucial intracellular messengers involved in numerous cellular processes.
Existing Ca2+ indicators may have limitations in specific applications, such as targeted protein labeling.
SNAP-tag technology allows for specific covalent labeling of proteins.

Purpose of the Study:

To develop a novel fluorescent Ca2+ indicator based on Fura-2FF.
To enable covalent linkage of the indicator to SNAP-tag fusion proteins.
To assess the Ca2+ sensing capabilities of the indicator after protein conjugation.

Main Methods:

Synthesis of a Fura-2FF derivative designed for conjugation.
Covalent coupling of the Fura-2FF indicator to SNAP-tag fusion proteins.
Characterization of fluorescence properties and Ca2+ binding affinity of the conjugated indicator.

Main Results:

Successful synthesis of the Fura-2FF-based fluorescent Ca2+ indicator.
Demonstration of covalent linkage to SNAP-tag fusion proteins.
Retention of Ca2+ sensing ability by the indicator after conjugation to proteins.

Conclusions:

A novel, protein-linkable fluorescent Ca2+ indicator has been developed.
This indicator can be used for specific labeling of SNAP-tag fusion proteins while retaining Ca2+ sensing function.
This tool offers new possibilities for studying Ca2+ dynamics in specific cellular compartments or protein interactions.