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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting.

Guillaume Mernier1, Niccolò Piacentini, Thomas Braschler

  • 1Laboratoire de Microsystèmes LMIS4, Ecole Polytechnique Fédérale de Lausanne, Station 17, CH-1015, Lausanne, Switzerland. guillaume.mernier@epfl.ch

Lab on a Chip
|June 18, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel device for electrical cell lysis and efficiency assessment in continuous flow. It achieves 99% yeast cell lysis efficiency using dielectrophoretic sorting, enabling high-throughput analysis.

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Area of Science:

  • Biotechnology
  • Electrical Engineering
  • Cell Biology

Background:

  • Cell lysis is a critical step in many biological and diagnostic processes.
  • Existing lysis methods can be inefficient, slow, or introduce contaminants.
  • Continuous flow systems offer advantages for high-throughput biological sample processing.

Purpose of the Study:

  • To develop and evaluate a device for efficient electrical cell lysis in a continuous flow system.
  • To assess lysis efficiency using dielectrophoretic cell sorting.
  • To investigate the potential throughput of the developed device.

Main Methods:

  • Utilized AC electrical fields and liquid electrodes to achieve cell lysis without bubble formation.
  • Employed numerical simulations to calculate electrical field distribution for optimal electrode configuration.
  • Integrated dielectrophoretic cell sorting to evaluate lysis efficiency by separating lysed and unlysed cells.

Main Results:

  • Demonstrated high lysis efficiency, achieving 99% sorting of yeast cells with dielectric properties indicative of lysis.
  • Successfully avoided bubble creation at electrode surfaces through the use of liquid electrodes.
  • Performed a study to assess the potential throughput of the device for continuous flow applications.

Conclusions:

  • The developed device offers an efficient and controllable method for electrical cell lysis in continuous flow.
  • Dielectrophoretic cell sorting provides a robust means to evaluate lysis efficiency.
  • The system shows promise for high-throughput biological sample preparation and analysis.