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Updated: Jun 12, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Proteomic analysis of mineralising osteoblasts identifies novel genes related to bone matrix mineralisation
Fawzy A Saad1, Jochen G Hofstaetter
1Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital Boston, Boston, MA, 02115, USA. fawzy.saad@tch.harvard.edu
Abstract:
Bone matrix mineralisation plays a critical role in the determination of the overall biomechanical competence of bone. However, the molecular mechanisms of bone matrix mineralisation have not been fully elucidated. We used a proteomic approach to identify proteins and genes that may play a role in osteoblast matrix mineralisation. Proteomic differential display revealed a protein band that appeared only in mineralising mouse 7F2 osteoblasts. In-gel protein digestion and mass spectrometry proteomic analysis of this protein band identified 16 proteins. Furthermore, their corresponding transcripts were upregulated. This identification of proteins that may be associated with bone matrix mineralisation presents important new information toward a better understanding of the precise mechanisms of this process.
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