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Related Experiment Videos

A new method for assaying antimicrobials in the prostate.

A C Philpott1, E D Crawford, G J Miller

  • 1University of Colorado Health Sciences Center-Division of Urology, Denver 80262.

Infection
|January 1, 1991
PubMed
Summary

This study measured antimicrobial concentrations in prostate tissue. Quinolones like norfloxacin and ciprofloxacin showed higher concentrations in prostate stroma compared to epithelium, exceeding E. coli MIC90s.

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Area of Science:

  • Urology
  • Pharmacology
  • Infectious Diseases

Background:

  • Prostate infections require effective antimicrobial penetration.
  • Understanding drug distribution within prostate tissue is crucial for treatment efficacy.

Purpose of the Study:

  • To quantify and compare intraprostatic concentrations of carbenicillin, norfloxacin, and ciprofloxacin.
  • To determine the distribution of these antimicrobials in prostate stroma versus epithelium.

Main Methods:

  • Prostatectomy specimens from 20 patients were analyzed.
  • High-Performance Liquid Chromatography (HPLC) was used to determine drug concentrations.
  • Stromal and epithelial compartments were separated for differential analysis.

Main Results:

Related Experiment Videos

  • Carbenicillin, norfloxacin, and ciprofloxacin concentrations were measured in prostate tissue.
  • Stromal to epithelial concentration ratios were calculated: carbenicillin (0.95), norfloxacin (1.28), ciprofloxacin (1.1).
  • Quinolones demonstrated a trend towards higher stromal concentrations, exceeding E. coli MIC90s significantly.

Conclusions:

  • Norfloxacin and ciprofloxacin achieve higher concentrations in prostate stroma than epithelium.
  • The tested quinolones achieve concentrations well above the MIC90 for E. coli in prostate tissue.
  • These findings support the potential efficacy of quinolones for treating prostate infections.