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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Identification of conserved features of LAGLIDADG homing endonucleases

Alexander Grishin1, Ines Fonfara, Andrei Alexeevski

  • 1AN Belozersky Institute for Physical and Chemical Biology, Moscow State University, Moscow 119991, Russia. grishin-a1@yandex.ru

Journal of Bioinformatics and Computational Biology
|June 18, 2010
PubMed

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Homologous Recombination

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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
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Conservation of Protein Domains Over Different Proteins

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Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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