Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Structural and compositional analyses of isolated cone matrix sheaths.

L V Johnson¹, G S Hageman

¹Department of Anatomy, University of Southern California, School of Medicine, Los Angeles 90033.

Investigative Ophthalmology & Visual Science

|June 1, 1991

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Levels of complement factor H-related 4 protein do not influence susceptibility to age-related macular degeneration or its course of progression.

Nature communications·2024

Same author

Use of neutralizing antibody test in diagnosis of human toxoplasmic choroiditis.

Archives of ophthalmology (Chicago, Ill. : 1929)·2010

Same author

Complement factor H gene polymorphisms and Chlamydia pneumoniae infection in age-related macular degeneration.

Eye (London, England)·2009

Same author

Individuals homozygous for the age-related macular degeneration risk-conferring variant of complement factor H have elevated levels of CRP in the choroid.

Proceedings of the National Academy of Sciences of the United States of America·2006

Same author

Variations in the complement regulatory genes factor H (CFH) and factor H related 5 (CFHR5) are associated with membranoproliferative glomerulonephritis type II (dense deposit disease).

Journal of medical genetics·2005

Same author

Apolipoprotein E allele-dependent pathogenesis: a model for age-related retinal degeneration.

Proceedings of the National Academy of Sciences of the United States of America·2005

The cone matrix sheath, a retinal layer, has a complex filamentous structure. This structure may mediate adhesion between the neural retina and retinal pigmented epithelium.

Area of Science:

Ophthalmology
Cell Biology
Biochemistry

Background:

The interphotoreceptor matrix (IPM) is crucial for photoreceptor function.
The cone matrix sheath (CMS) is a distinct IPM domain associated with cone photoreceptors.

Purpose of the Study:

To develop a method for isolating CMS-enriched fractions.
To investigate the structure and composition of the CMS.

Main Methods:

Aqueous extraction technique for CMS isolation.
Gel electrophoresis and lectin blot analysis.

Main Results:

CMS exhibits a complex filamentous substructure with longitudinal filaments and anastomosing networks.
Filamentous rings at apical and basal ends connect CMS structures.

Related Experiment Videos

Peanut lectin-labeled 17- and 32-kilodalton proteins were identified in CMS fractions.

Basal rings of CMS interconnect with rod photoreceptor matrix material.

Conclusions:

The CMS possesses structural elements potentially mediating adhesion between the neural retina and retinal pigmented epithelium.
These structural components may explain the firm adherence of CMS to retinal layers.